2A Peptide Antibody (3H4) - BSA Free Best Seller
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59627
Non-recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Non-species specific
Cited:
Human, Mouse, Hamster - Cricetulus (Chinese Hamster)
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3H4
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This 2A Peptide Antibody (3H4) was developed against KLH-coupled synthetic peptide corresponding to 2A peptide of Thosea asigna virus (CGDVEENPG)
Specificity
This 2A Peptide Antibody (3H4) peptide antibody is specific for T2A and P2A tagged proteins. Specificity to F2A and E2A proteins has not been tested.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for 2A Peptide Antibody (3H4) - BSA Free
Western Blot Detection of 2A Peptide in HeLa Cells
Total protein from SpCas9-T2A peptide transfected HeLa cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-T2A in 5% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Detection of 2A Peptide in NIH-3T3 Cells
2A Peptide Antibody used for Western Blotting. NIH-3T3 cells were stably integrated with a lentiviral plasmid expressing a fluorescent protein-P2A protein. Two different viral titers were used in this experiment. Image from verified customer review.Applications for 2A Peptide Antibody (3H4) - BSA Free
Application
Recommended Usage
Western Blot
1 ug/ml
Application Notes
Use in flow cytometry reported in PMID: 36356599 and customer review. Novus does not validate this product in flow cytometry and is unable to guarantee its performance.
Reviewed Applications
Read 2 reviews rated 4.5 using NBP2-59627 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: 2A Peptide
Developed for genetic engineering, 2A cleavage has been shown to function in a wide range of eukaryotic cells, and in vivo (3). The 2A peptide is inserted between the coding sequences of at least two genes, enabling the simultaneous expression of multiple proteins from a single plasmid construct. Four variants of 2A peptides have been used in gene expression systems including FMDV 2A (F2A), equine rhinitis A virus 2A (E2A), porcine teschovirus-1 2A (P2A) or Thoseaasigna virus 2A (T2A). Compared to other multi-gene co-expression systems such as IRES, 2A peptides results in stoichiometric expression of different proteins. However, cleaving efficiency and protein expression using the different 2A peptides varies, requiring optimization of polycistronic constructs for experiments.
References
1. Liu Z, Chen O, Wall J, Zheng M, Zhou Y, Wang L, Vaseghi H, Qian L, Liu J. (2017). Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector. Scientific Reports. 7. 10.1038/s41598-017-02460-2.
2. Luke GA, Escuin H, Felipe PD, Ryan MD. (2009) 2A to the Fore - Research, Technology and Applications, Biotechnology and Genetic Engineering Reviews. 26(1): 223-260.
3. Markus BM, Bell GW, Lorenzi HA, Lourido S. (2019) Optimizing Systems for Cas9 Expression in Toxoplasma gondii. mSphere. 4(3): e00386-19.
Alternate Names
2A cleavage peptide, 2A peptide, 2A Peptide Antibody, 2A peptide tag, 2A sequence, 2A-like peptide, antibody for 2A-containing recombinant proteins, FMDV 2A-peptide, FMDV 2A-peptide antibody, MDV 2A-peptide, P2A peptide, P2A tagged proteins, peptide 2A sequences, self-cleaving 2A peptide, T2A peptide, T2A tagged proteins, Thosea asigna virus 2A, Thosea asigna virus 2A antibody
Additional 2A Peptide Products
Product Documents for 2A Peptide Antibody (3H4) - BSA Free
Product Specific Notices for 2A Peptide Antibody (3H4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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