Western blot shows lysates of human kidney tissue, human placenta tissue, and human colon tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human 11 beta-HSD2 Monoclonal Antibody (Catalog # MAB8630) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 11 beta-HSD2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

11 beta‑HSD2 in Human Kidney.

11 beta-HSD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human 11 beta-HSD2 Monoclonal Antibody (Catalog # MAB8630) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human 11 beta-HSD2 by Simple Western^TM.

Left: Simple Western lane view shows lysates of human placenta, loaded at 1 mg/ml. A specific band was detected for 11 beta-HSD2 at approximately 46 kDa (as indicated) using both 2 µg/ml and 10 µg/ml of Mouse Anti-Human 11 beta-HSD2 Monoclonal Antibody (Catalog # MAB8630) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Mouse Anti-Human 11 beta-HSD2 Monoclonal Antibody (Catalog # MAB8630) tested at 2 µg/ml (blue line) and 10 µg/ml (green line) in the human placenta.

Applications for Human 11 beta-HSD2 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Simple Western

2-10 µg/mL
Sample: Human placenta

Western Blot

1 µg/mL
Sample: Human kidney tissue, human placenta tissue, and human colon tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 11 beta-HSD2

The human enzyme HSD-2/11 beta-HSD2 is encoded by the HSD11B2 gene and codes for a 405 aa protein, with high expression in kidney, colon, pancreas and placenta1. HSD-2 modulates intracellular glucocorticoid levels by catalyzing the conversion of cortisol to the inactive metabolite cortisone2. HSD-2 is abundantly expressed in human placenta and controls fetal glucocorticoid levels to protect the fetus from glucocorticoid exposure. Similarly, HSD-2 expression in the distal nephron protects mineralcorticoid receptors from glucocorticoids and helps regulate blood pressure3. Exposure to major environmental pollutant like Cadmium, inhibits activity of HSD-2 which affects the synthesis of glucocorticoids in placenta and thus contribute to developing preeclamptic conditions.

Long Name

11 beta Hydroxysteroid Dehydrogenase 2

Alternate Names

11 betaHSD2, HSD11B2

Entrez Gene IDs

3291 (Human); 15484 (Mouse); 25117 (Rat)

Gene Symbol

HSD11B2

UniProt

P80365

Additional 11 beta-HSD2 Products

Product Documents for Human 11 beta-HSD2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human 11 beta-HSD2 Antibody

For research use only

Citations
Reviews

Human 11 beta-HSD2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8630

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human 11 beta-HSD2 Antibody

Detection of Human 11 beta-HSD2 by Western Blot.