Human/Mouse/Rat 11 beta-HSD1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3397
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat, Rabbit
Applications
Validated:
Western Blot, Simple Western
Cited:
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human 11 beta-HSD1
Asn24-Lys292
Accession # P28845
Asn24-Lys292
Accession # P28845
Specificity
Detects endogenous human, mouse and rat 11 beta-HSD1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat 11 beta-HSD1 Antibody
Detection of Human/Mouse/Rat 11 beta-HSD1 by Western Blot.
Western blot shows lysates of human, mouse and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat 11 beta-HSD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3397) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for 11 beta-HSD1 at approximately 36 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Detection of Human 11 beta-HSD1 by Simple WesternTM.
Left: Simple Western lane view shows lysates of human liver, loaded at 1 mg/ml. A specific band was detected for 11 beta-HSD1 at approximately 48 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Goat Anti-Human/Mouse/Rat 11 beta-HSD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3397) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # 043-522-2). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Goat Anti-Human/Mouse/Rat 11 beta-HSD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3397) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the human liver.
Detection of 11 beta-HSD1 by Western Blot
Effect of S100A16 on 11 beta-HSD1 expression Adipose, liver, and muscle tissues were removed from C57BL/6, S100A16Tg/Tg, and S100A16KO/+ mice, and proteins were extracted. (A) Analysis of S100A16 and 11 beta-HSD1 protein levels by Western blotting. (B) Relative expression of S100A16 and 11 beta-HSD1 based on grayscale analysis with alpha-tubulin as a control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31399502), licensed under a CC-BY license. Not internally tested by R&D Systems.
Applications for Human/Mouse/Rat 11 beta-HSD1 Antibody
Application
Recommended Usage
Simple Western
10-50 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Western Blot
0.5 µg/mL
Sample: Human, mouse and rat liver tissue
Sample: Human, mouse and rat liver tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 11 beta-HSD1
Hydoxysteroid (11-beta) Dehydrogenase, type I 11-beta-hydroxysteroid dehydrogenase 1(11 beta-HSD1, HSD11B1 or HSD1) is a microsomal glycoprotein enzyme, with an apparent mass of 36 kDa, that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. 11 beta-HSD1 can also catalyze the reverse reaction, the conversion of cortisone to cortisol. 11 beta-HSD1 has been detected in a wide range of rat and human tissues, including lung and testis, but highest amounts are expressed in the liver. Human 11 beta-HSD1 shares 79 and 77% sequence identity with mouse and rat 11 beta-HSD1, respectively. There are at least 2 isoforms of 11 beta-HSD. The other isoform, type II, is expressed predominantly in the kidney and placenta and catalyzes only the 11-beta-dehydrogenation reaction. High levels of cortisol can lead to visceral obesity and particular defects in the expression or function of 11 beta-HSD1 have also been associated with insulin resistance and cognitive function.
Long Name
11 beta Hydroxysteroid Dehydrogenase 1
Alternate Names
11 betaHSD1, HSD11B1
Gene Symbol
HSD11B1
UniProt
Additional 11 beta-HSD1 Products
Product Documents for Human/Mouse/Rat 11 beta-HSD1 Antibody
Product Specific Notices for Human/Mouse/Rat 11 beta-HSD1 Antibody
For research use only
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