4‑Hydroxynonenal Antibody Best Seller
Catalog # MAB3249 | R&D Systems, Inc. a Bio-Techne Brand
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Product Summary for 4‑Hydroxynonenal Antibody
Immunogen
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Scientific Data Images for 4‑Hydroxynonenal Antibody
Detection of Human 4‑Hydroxynonenal by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE). PVDF membrane was probed with 1 µg/mL of Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for 4-Hydroxynonenal at adducts of histidine residues (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Detection of Human 4‑Hydroxynonenal by Simple WesternTM.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE), loaded at 0.2 mg/mL. Specific bands were detected for 4‑Hydroxynonenal at adducts of histidine residues kDa (as indicated) using 5 µg/mL of Mouse Anti-4‑Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.4‑Hydroxynonenal in Human Prostate.
4-Hydroxynonenal was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for 4‑Hydroxynonenal Antibody
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of human normal prostate and prostate cancer tissue
Simple Western
Sample: HepG2 human hepatocellular carcinoma cell line treated with 4-Hydroxynonenal (4-HNE)
Western Blot
Sample: HepG2 human hepatocellular carcinoma cell line treated with 4-Hydroxynonenal (4-HNE)
Reviewed Applications
Read 3 reviews rated 4.3 using MAB3249 in the following applications:
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Reconstitution
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Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 4-Hydroxynonenal
Alternate Names
Product Documents for 4‑Hydroxynonenal Antibody
Product Specific Notices for 4‑Hydroxynonenal Antibody
For research use only
Citations for 4‑Hydroxynonenal Antibody (36)
Citations are publications that use Bio-Techne products. Selected citations for 4‑Hydroxynonenal Antibody include:
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Species: Mouse
Sample Types: Cell Lysates,Whole Tissue
Applications: Western Blot,IHCYJ Kahm et al. (2023-04-06), RanBP1: A Potential Therapeutic Target for Cancer Stem Cells in Lung Cancer and Glioma International Journal of Molecular Sciences, 2023-024(7).
PMID: 37047826 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCYJ Chiu et al. (2023-01-30), Investigating Therapeutic Effects of Indole Derivatives Targeting Inflammation and Oxidative Stress in Neurotoxin-Induced Cell and Mouse Models of Parkinson's Disease International Journal of Molecular Sciences, 2023-024(3).
PMID: 36768965 -
Species: Rat
Sample Types: Tissue Lysates
Applications: Western BlotSCW Sze et al. (2022-10-21), Aberrant Transferrin and Ferritin Upregulation Elicits Iron Accumulation and Oxidative Inflammaging Causing Ferroptosis and Undermines Estradiol Biosynthesis in Aging Rat Ovaries by Upregulating NF-kappab-Activated Inducible Nitric Oxide Synthase: First Demonstration of an Intricate Mechanism International Journal of Molecular Sciences, 2022-123(20).
PMID: 36293552 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCP Siriviriya et al. (2022-08-04), Genistein attenuated oxidative stress, inflammation, and apoptosis in L-arginine induced acute pancreatitis in mice BMC complementary medicine and therapies, 2022-022(1):208.
PMID: 35927726 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCN Li et al. (2022-06-21), Targeting Ferroptosis as a Novel Approach to Alleviate Aortic Dissection Oncogene, 2022-018(10):4118-4134.
PMID: 35844806 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHC/IFC Liu et al. (2022-03-18), Glia maturation factor-beta induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy Redox Biology, 2022-052(0):102292.
PMID: 35325805 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCM Baškovi? et al. (2022-02-26), Astaxanthin Relieves Testicular Ischemia-Reperfusion Injury-Immunohistochemical and Biochemical Analyses Journal of Clinical Medicine, 2022-011(5).
PMID: 35268375 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCG Dang et al. (2022-02-04), T lymphocyte-derived extracellular vesicles aggravate abdominal aortic aneurysm by promoting macrophage lipid peroxidation and migration via pyruvate kinase muscle isozyme 2 Redox Biology, 2022-050(0):102257.
PMID: 35149342 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCHH Dieguez et al. (2021-12-04), Enriched environment and visual stimuli protect the retinal pigment epithelium and photoreceptors in a mouse model of non-exudative age-related macular degeneration Cell Death & Disease, 2021-112(12):1128.
PMID: 34864827 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCD Das et al. (2021-06-11), Proteasome dysfunction under compromised redox metabolism dictates liver injury in NASH through ASK1/PPAR&gamma binodal complementary modules Redox Biology, 2021-045(0):102043.
PMID: 34144391 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCJ Misra et al. (2021-06-04), Discordant regulation of eIF2 kinase GCN2 and mTORC1 during nutrient stress Nucleic Acids Research, 2021-049(10):5726-5742.
PMID: 34023907 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCZ Fang et al. (2021-05-04), Sestrin proteins protect against lipotoxicity-induced oxidative stress in the liver via suppression of c-Jun N-terminal kinases: Short title: Sestrins ameliorate hepatic lipotoxicity Cellular and Molecular Gastroenterology and Hepatology, 2021-00(0).
PMID: 33962074 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotCS Bruells et al. (2021-03-18), Acute liver injury following acetaminophen administration does not activate atrophic pathways in the mouse diaphragm Scientific Reports, 2021-011(1):6302.
PMID: 33737702 -
Species: Mouse
Sample Types: Tissue
Applications: IHC-PM Li et al. (2020-04-12), Sirt3 modulates fatty acid oxidation and attenuates cisplatin-induced AKI in mice J. Cell. Mol. Med., 2020-00(0).
PMID: 32281286 -
Species: Mouse
Sample Types: Cell Lystates
Applications: Western BlotH Tendilla-B et al. (2019-09-13), Risperidone ameliorates prefrontal cortex neural atrophy and oxidative/nitrosative stress in brain and peripheral blood of rats with neonatal ventral hippocampus lesion J. Neurosci., 2019-00(0).
PMID: 31519825 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotP Siriviriya et al. (2019-08-27), Effects of curcumin on oxidative stress, inflammation and apoptosis in L-arginine induced acute pancreatitis in mice Heliyon, 2019-05(8):e02222.
PMID: 31485503 -
Species: Ovine
Sample Types: Tissue Homogenates
Applications: Western BlotF Liang et al. (2019-04-16), Mechanical ventilation causes diaphragm dysfunction in newborn lambs Crit Care, 2019-023(1):123.
PMID: 30992039 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotNG Barra et al. (2018-07-01), Maternal Nicotine Exposure (MNE) leads to augmented expression of the anti-oxidant adipose tissue triglyceride lipase (ATGL) long-term in the white adipose of female rat offspring Toxicol. Sci., 2018-00(0).
PMID: 29617909 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotH Lee et al. (2018-03-21), Tocotrienol-rich fraction supplementation reduces hyperglycemia-induced skeletal muscle damage through regulation of insulin signaling and oxidative stress in type 2 diabetic mice J. Nutr. Biochem., 2018-057(0):77-85.
PMID: 29679925 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrA Chatterjee et al. (2018-01-25), The Addition of Manganese Porphyrins during Radiation Inhibits Prostate Cancer Growth and Simultaneously Protects Normal Prostate Tissue from Radiation Damage Antioxidants (Basel), 2018-07(1).
PMID: 29370088 -
Species: Rat
Sample Types: Whole Tissue
Applications: Western BlotJM Gemechu et al. (2018-01-24), Characterization of Dopaminergic System in the Striatum of Young Adult Park2-/- Knockout Rats Sci Rep, 2018-08(1):1517.
PMID: 29367643 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotJ Zhao et al. (2017-11-30), Age-dependent increase in angiopoietin-like protein 2 accelerates skeletal muscle loss in mice J. Biol. Chem., 2017-10(0).
PMID: 29191837 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotWS Hambright et al. (2017-02-01), Ablation of ferroptosis regulator glutathione peroxidase 4 in forebrain neurons promotes cognitive impairment and neurodegeneration Redox Biol, 2017-012(0):8-17.
PMID: 28212525 -
Species: Mouse
Sample Types: Plasma
Applications: Western BlotJ Shin et al. (2016-12-13), Gamma-tocopherol supplementation ameliorated hyper-inflammatory response during the early cutaneous wound healing in alloxan-induced diabetic mice Exp. Biol. Med. (Maywood), 2016-1242(5):505-515.
PMID: 28211759 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotJ Seo et al. (2016-04-25), Oxidative Stress Triggers Body-Wide Skipping of Multiple Exons of the Spinal Muscular Atrophy Gene PLoS ONE, 2016-011(4):e0154390.
PMID: 27111068 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotGarate I et al. (2014-01-11), Toll-like 4 receptor inhibitor TAK-242 decreases neuroinflammation in rat brain frontal cortex after stress. J Neuroinflammation, 2014-011(0):8.
PMID: 24410883 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotHolloway et al. (2013-12-24), Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies. Am J Physiol Endocrinol Metab, 2013-1306(4):E443-56.
PMID: 24368670 -
Species: Drosophila
Sample Types: Whole Tissue
Applications: IHCBufalino et al. (2013-05-06), The asymmetric segregation of damaged proteins is stem cell-type dependent. J Cell Biol, 2013-0201(4):523-30.
PMID: 23649805 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotMargaritis M et al. (2013-04-26), Interactions between vascular wall and perivascular adipose tissue reveal novel roles for adiponectin in the regulation of endothelial nitric oxide synthase function in human vessels. Circulation, 2013-0127(22):2209-21.
PMID: 23625959 -
Species: Mouse
Sample Types: Tissue Homogenates,Whole Tissue
Applications: Western Blot,IHC(2012-10-23), Anti-inflammatory and neuroprotective effects of an orally active apocynin derivative in pre-clinical models of Parkinson's disease. J Neuroinflammation, 2012-19(0):241.
PMID: 23092448 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot(2012-06-14), Transgenic control of mitochondrial fission induces mitochondrial uncoupling and relieves diabetic oxidative stress. Diabetes, 2012-061(8):2093-104.
PMID: 22698920 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotWang Z et al. (2011-11-10), 4-Hydroxynonenal differentially regulates adiponectin gene expression and secretion via activating PPARgamma and accelerating ubiquitin-proteasome degradation. Mol. Cell. Endocrinol., 2011-1349(2):222-31.
PMID: 22085560 -
Species: Rat
Sample Types: Cell Lysates
Applications: Western BlotWalson KH et al. (2011-02-01), Normoxic versus hyperoxic resuscitation in pediatric asphyxial cardiac arrest: effects on oxidative stress. Crit. Care Med., 2011-039(2):335-43.
PMID: 21057313 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotYano M et al. (2010-11-29), Mitochondrial dysfunction and increased reactive oxygen species impair insulin secretion in sphingomyelin synthase 1-null mice. J. Biol. Chem., 2010-1286(5):3992-4002.
PMID: 21115496 -
Species: Rat
Sample Types: Whole Cells
Applications: ICCStraface E et al. (2009-10-03), Cell sex determines anoikis resistance in vascular smooth muscle cells. FEBS Lett., 2009-1583(21):3448-54.
PMID: 19804776 -
Species: Human
Sample Types: Whole Cells
Applications: ICCMargutti P et al. (2007-11-09), Autoantibodies to the C-terminal subunit of RLIP76 induce oxidative stress and endothelial cell apoptosis in immune-mediated vascular diseases and atherosclerosis. Blood, 2007-1111(9):4559-70.
PMID: 17993611
There are no citations that match your criteria.
Customer Reviews for 4‑Hydroxynonenal Antibody (3)
Customer Images
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Application: ImmunohistochemistrySample Tested: Liver tissueSpecies: MouseVerified Customer | Posted 08/11/2021
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Aortic valvesSpecies: MouseVerified Customer | Posted 03/29/2021-Fixed in 10% neutral buffered formalin followed by dehydration process -10 um thick cryosection - Blocking: Normal Goat Serum (NGS) - Primary Ab (4-HNE): diluted in NGS at 20 ug/mL; 4C overnight - Secondary (AF488): 1:100 dilution in NGS; room temp 1hr - Vector anti-fade mounting media with DAPI
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Application: Western BlotSample Tested: See PMID 21429624Species: MouseVerified Customer | Posted 02/19/2015
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FAQs for 4‑Hydroxynonenal Antibody
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