R&D Systems offers reagents to promote epitope availability and enhance immunoreactivity. Antigen Retrieval Reagent-Basic is an antigen retrieval system that utilizes heat-induced recovery of cell and tissue antigens. The Basic solution included in the kit may also affect tissue morphology. The optimal antigen unmasking condition is a function of temperature (90 - 100 °C) and incubation time (up to 30 minutes), and should be established by each individual investigator.
Neuroplastin 65 was detected in human cerebellar tissue using 3 μg/mL goat anti-human/mouse Neuroplastin 65 polyclonal antibody (Catalog # AF5360). Tissue was pretreated with R&D Systems Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using R&D Systems Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown, Catalog # CTS008) and counterstained with hematoxylin (blue).
Preparation & Storage
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at room temperature. Do not use past expiration date.