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Mouse IgG Horseradish Peroxidase-conjugated Antibody Best Seller

Catalog # HAF007 | R&D Systems, Inc. a Bio-Techne Brand
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HAF007

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

HRP

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse IgG Horseradish Peroxidase-conjugated Antibody

Immunogen

Mouse IgG

Specificity

Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with human IgG and rabbit IgG is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse IgG Horseradish Peroxidase-conjugated Antibody

Detection of Mouse IgG Primary Antibody by Western Blot

Detection of Mouse IgG Primary Antibody by Western Blot

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Myocilin in human skeletal muscle

Detection of Myocilin in human skeletal muscle using 5 ug/mL of Mouse monoclonal anti-human Myocilin primary antibody (MAB3446) followed by 30 min incubation with Goat anti Mouse HRP conjugated secondary antibodies (Catalog # HAF007) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.

Applications for Mouse IgG Horseradish Peroxidase-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Use at a 1:20 dilution 

Western Blot

Use at a 1:1000 dilution.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 15 reviews rated 4.7 using HAF007 in the following applications:

Published Applications

Read 124 publications using HAF007 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Formulation

Sodium Phosphate and Proclin 300.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG, IGHG1, Immunoglobulin G, ImmunoglobulinG

Product Documents for Mouse IgG Horseradish Peroxidase-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IgG Horseradish Peroxidase-conjugated Antibody

For research use only

Citations for Mouse IgG Horseradish Peroxidase-conjugated Antibody (124)

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Customer Reviews for Mouse IgG Horseradish Peroxidase-conjugated Antibody (15)

4.7 out of 5
15 customer ratings
5 stars
80%
4 stars
13%
3 stars
7%
2 stars
0%
1 star
0%

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Showing  1 - 5 of 15 reviews Showing All
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  • Mouse IgG Horseradish Peroxidase-conjugated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: mouse serum
    Species: Mouse
    Verified Customer | Posted 08/12/2023
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG Horseradish Peroxidase-conjugated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 08/12/2023
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 03/03/2023
    stable and reproducible
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Mouse
    Verified Customer | Posted 07/15/2021
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Mouse
    Verified Customer | Posted 04/29/2021
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293T and NIH3T3 whole cell lysate
    Species: Human
    Verified Customer | Posted 04/15/2021
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line and NIH-3T3 mouse embryonic fibroblast cell line
    Species: Human
    Verified Customer | Posted 04/12/2021
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mv1Lu mink lung epithelial cell line
    Species: Mink
    Verified Customer | Posted 11/20/2019
    used as secondary antibody for WB at 1:1000 with 1hr incubation at RT
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Danlee Enzler
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Liver cancer tissue
    Species: Mouse
    Verified Customer | Posted 05/01/2018
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 04/16/2018
    We are using this product for Elisa assay for various human samples, we used at 1:1000 dilution, and it works very well, no artificial background.
  • Mouse IgG HRP-conjugated Antibody
    Name: Stephanie Watkins
    Application: Western Blot
    Sample Tested: T cells
    Species: Human
    Verified Customer | Posted 02/22/2018
    Mouse anti-human beta Actin was used as primary and then R&D Systems goat-anti mouse was used a a secondary (Incubated for one hour at RT in 5% BSA). Membrane was developed using ECL substrate.
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: antibody
    Species: Goat
    Verified Customer | Posted 10/20/2017
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human and Mouse
    Verified Customer | Posted 06/28/2017
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG Horseradish Peroxidase-conjugated Antibody
    Name: Alexandria Schieber
    Application: Western Blot
    Sample Tested: Small intestine and Adipose tissue
    Species: Mouse
    Verified Customer | Posted 02/22/2016
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG Horseradish Peroxidase-conjugated Antibody
    Name: Jaiyanth Daniel
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: E. coli
    Verified Customer | Posted 02/22/2016
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007

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Showing  1 - 5 of 15 reviews Showing All
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