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Mouse IgG1 PE‑conjugated Antibody

Catalog # IC002P | R&D Systems, Inc. a Bio-Techne Brand
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IC002P

Key Product Details

Species Reactivity

Mouse

Applications

Control

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 11711

Product Summary for Mouse IgG1 PE‑conjugated Antibody

Immunogen

KLH

Specificity

Selected for use as mouse IgG1 isotype control for flow cytometry applications.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Mouse IgG1 PE‑conjugated Antibody

Detection of Mouse IgG Isotype Control by Flow Cytometry

Detection of Mouse IgG Control by Flow Cytometry

A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human RANK/TNFRSF11A PE-conjugated Monoclonal Antibody (Catalog # FAB683P, filled histogram) or Mouse IgG PE-conjugated Isotype Control Antibody (Catalog # IC002P, open histogram).

Applications for Mouse IgG1 PE‑conjugated Antibody

Application
Recommended Usage

Control

Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 11 reviews rated 4.9 using IC002P in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, IGHG1, Igh-4, RGD1359539, VH7183

Product Documents for Mouse IgG1 PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IgG1 PE‑conjugated Antibody

For research use only

Citations for Mouse IgG1 PE‑conjugated Antibody (61)

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Customer Reviews for Mouse IgG1 PE‑conjugated Antibody (11)

4.9 out of 5
11 customer ratings
5 stars
91%
4 stars
9%
3 stars
0%
2 stars
0%
1 star
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  • Mouse IgG1 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Mouse spleenocytes
    Species: Mouse
    Verified Customer | Posted 06/08/2022
  • Mouse IgG1 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: PC-3 human prostate cancer cell line
    Species: Human
    Verified Customer | Posted 06/07/2022
    good control.
  • Mouse IgG1 PE-conjugated Antibody
    Name: Anonymous
    Verified Customer | Posted 12/14/2020
    Mouse IgG1 PE‑conjugated Antibody IC002P
  • Mouse IgG1 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 07/26/2020
    Mouse IgG1 PE‑conjugated Antibody IC002P
  • Mouse IgG1 PE-conjugated Antibody
    Name: Boubacar Barry
    Application: ELISA
    Sample Tested: 2120Ep human embryonal carcinoma cell line
    Species: Human
    Verified Customer | Posted 08/11/2017
  • Mouse IgG1 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood-derived monocytes
    Species: Human
    Verified Customer | Posted 08/01/2017
  • Mouse IgG1 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: U937 human histiocytic lymphoma cell line
    Species: Human
    Verified Customer | Posted 06/29/2017
    Mouse IgG1 PE‑conjugated Antibody IC002P
  • Mouse IgG1 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human cell line
    Species: Human
    Verified Customer | Posted 03/01/2017
    FEMX-I Human melanoma cells (10^6) were incubated for 30 minutes at 4ºC with 10 ul of Mouse anti-Human CD9 PE-conjugated antibody (red), or Mouse Isotype Control IgG1-PE (R&D #IC002P, black).
    Mouse IgG1 PE‑conjugated Antibody IC002P
  • Mouse IgG1 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: iDC human immature myeloid dendritic cells
    Species: Human
    Verified Customer | Posted 09/08/2016
    Mouse IgG1 PE‑conjugated Antibody IC002P
  • Mouse IgG1 PE-conjugated Antibody
    Name: Concepcion Estaras
    Application: Flow Cytometry
    Sample Tested: H1 human embryonic stem cells treated with GSK3i
    Species: Human
    Verified Customer | Posted 06/15/2016
    H1 cells were treated with ChIR for 48h. 1x106 cells were incubated with 10ul of Mouse IgG1 PE-conjugated (left image) or KDR-PE antibody (right image) for 45min at 4C. Cell were then analyzed using the LSRII analyzer.
  • Mouse IgG1 PE-conjugated Antibody
    Name: Aida Flores
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 05/17/2016

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