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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 539730

Product Specifications

Immunogen

E. coli-derived recombinant human Pygopus-2
Met160-Glu336
Accession # NP_612157

Specificity

Detects human Pygopus-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Pygopus-1 or recombinant mouse Pygopus-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Pygopus-2 Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Pygopus-2

Human Pygopus-2 (pygopus; the name of a legless Australian lizard) is a 41 kDa, 406 amino acid (aa) transcriptional coactivator.  It contains three domains: an N‑terminal NLS (aa 41-47), followed by a proline-rich region and a zinc-finger PHD-type domain (aa 330-387).  A potential internal alternate start site may generate an isoform that deletes the first 37 aa.  Pygopus 2 is a nuclear protein that acts in concert with BCL-9 and TCF to retain beta-Catenin in the nucleus during Wnt-signaling.  It does so by forming a “chain-of-adaptors” with the above three proteins.  Over the range of aa used for immunization, human pygopus 2 is 95% identical to mouse.

Alternate Names

PYGO2, Pygopus2

Entrez Gene IDs

90780 (Human); 68911 (Mouse)

Gene Symbol

PYGO2

UniProt

Additional Pygopus-2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Pygopus-2 Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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