Human Pro Collagen I alpha 1 Alexa Fluor® 350-conjugated Antibody

Name: Human Pro Collagen I alpha 1 Alexa Fluor® 350-conjugated Antibody
Brand: R&D Systems
SKU: FAB6220U

R&D Systems, part of Bio-Techne | Catalog # FAB6220U

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Product Datasheet / COA

Key Product Details

Species Reactivity:	Human
Immunogen:	Chinese hamster ovary cell line CHO-derived recombinant human Collagen I Gln23-Lys277, Gly1094-Leu1464 Accession # P02452
Applications:	Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label:	Alexa Fluor 350 (Excitation=346 nm, Emission=442 nm)
Specificity:	Detects human Pro-Collagen I alpha 1 in direct ELISAs.

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Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunoprecipitation	Optimal dilution of this antibody should be experimentally determined.
Immunocytochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: Collagen I alpha 1

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides (1). The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues (1). Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described (2). This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N‑telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I( alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.

Item	Value
Entrez Gene IDs	1277 (Human); 12842 (Mouse); 29393 (Rat)
Alternate Names	Alpha-1 type I collagen, COL-IA1, COL1A1, Collagen 1, Collagen 1 alpha 1, Collagen I alpha 1, Collagen Type I Alpha 1 Chain, Collagen-1, Collagen1, EDSARTH1, OI4, Type I Procollagen Alpha 1 Chain, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, pro-alpha-1 collagen type 1

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