Human PREB Alexa Fluor® 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5557V
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PREB
Met1-Ser388
Accession # Q9HCU5
Met1-Ser388
Accession # Q9HCU5
Specificity
Detects human PREB in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human PREB Alexa Fluor® 405-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: PREB
PREB (Prolactin regulatory element-binding protein; also mSEC12) is a 45 kDa member of the WD-repeat protein family. Although it is ubiquitously expressed, its expression level varies, tissue-to-tissue. It apparently acts as both a DNA-binding transcription factor, and a catalyst for vesicle formation from the ER. Human PREB is a 417 amino acid (aa) type II transmembrane protein. It is found in the ER and nuclear envelope, and may undergo splicing to generate a transcriptional factor. PREB contains a large cytoplasmic region (aa 1‑388) that displays three WD repeats (aa 152‑337), and an eight aa lumenal domain (aa 410‑417). The transcription factor region lies between aa 175‑417. Multiple splice forms exist. There is an alternate start site at Met73 that may be accompanied by either a 47 aa substitution for aa 388‑417, or a 14 aa substitution for aa 409‑417. Over aa 1‑388, human PREB shares 88% aa identity with mouse PREB.
Long Name
Prolactin Regulatory Element Binding
Alternate Names
MGC3467, SEC12
Gene Symbol
PREB
UniProt
Additional PREB Products
Product Documents for Human PREB Alexa Fluor® 405-conjugated Antibody
Product Specific Notices for Human PREB Alexa Fluor® 405-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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