Detects human Podocalyxin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Podocalyxin or recombinant human Endoglycan is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human Podocalyxin PE-conjugated Antibody

Detection of Podocalyxin in MDA-MB-231 human cell line by Flow Cytometry.

MDA-MB-231 (orange) or MG-63 (blue) cell lines were stained with Mouse Anti-Human Podocalyxin PE-conjugated Monoclonal Antibody (Catalog # FAB1658P, filled histograms) or isotype control antibody (Catalog # IC003P, open histograms). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Podocalyxin PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

2.5 µg/mL
Sample: MDA-MB-231 Human Cell Line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

References

Li, J. et al. (2001) DNA Seq. 12:407.
Hara, T. et al. (1999) Immunity 11:567.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Entrez Gene IDs

5420 (Human); 27205 (Mouse)

Gene Symbol

PODXL

UniProt

AAB61574

Additional Podocalyxin Products

Product Documents for Human Podocalyxin PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Podocalyxin PE-conjugated Antibody

For research use only

Citations
Reviews

Human Podocalyxin PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1658P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Podocalyxin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Podocalyxin PE-conjugated Antibody

Detection of Podocalyxin in MDA-MB-231 human cell line by Flow Cytometry.

Applications for Human Podocalyxin PE-conjugated Antibody

Flow Cytometry

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: Podocalyxin

References

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Podocalyxin Products

Product Documents for Human Podocalyxin PE-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Human Podocalyxin PE-conjugated Antibody