Human PLC‑ beta 3 Alexa Fluor® 532-conjugated Antibody

Name: Human PLC‑ beta 3 Alexa Fluor® 532-conjugated Antibody
Brand: R&D Systems
SKU: AF4716X

R&D Systems, part of Bio-Techne | Catalog # AF4716X

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Product Datasheet / COA

Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human PLC-Î²3 Lys27-Leu246 Accession # Q01970
Applications:	Western Blot
Label:	Alexa Fluor 532 (Excitation=534 nm, Emission=553 nm)
Specificity:	Detects human PLC-beta 3 in Western blots. Does not detect recombinant human (rh) PLC-beta 1, rhPLC-beta 2, or rhPLC-beta 4 in Western blots.

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Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: PLC-beta 3

The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-beta 3 (Phospholipase C beta-3) is a G-protein dependent phosphodiesterase that catalyzes the generation of inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) from phosphatidylinositol 4,5-bisphosphate (IP2), an essential step in the intracellular transduction of many extracellular signals. The PLC-beta subfamily consists of 4 isozymes, beta 1‑4, which differ in their tissue distribution and their ability to be activated by G proteins. PLC-beta 1 and PLC-beta 3 are more widely expressed, whereas PLC-beta 2 is resticted to hematopoietic cells and PLC-beta 4 is limited to certain neuronal cells and the retina.

Item	Value
Long Name	Phospholipase C beta 3
Entrez Gene IDs	5331 (Human); 18797 (Mouse); 29322 (Rat)
Alternate Names	1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3, EC 3.1.4.11, FLJ37084, PLC beta 3, PLC-beta 3, PLC-beta-3, PLCB3, PLCbeta 3, Phosphoinositide phospholipase C-beta-3, Phospholipase C-beta-3, phospholipase C, beta 3 (phosphatidylinositol-specific)

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