Detects human PIWIL1/HIWI in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) PIWIL2 or rhPIWIL4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human PIWIL1/HIWI Antibody

PIWIL1/HIWI in NCI‑N87 Human Cell Line.

PIWIL1/HIWI was detected in immersion fixed NCI-N87 human gastric carcinoma cell line using Mouse Anti-Human PIWIL1/HIWI Monoclonal Antibody (Catalog # MAB6548) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm in colonies of NCI-N87 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human PIWIL1/HIWI Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NCI-N87 human gastric carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PIWIL1/HIWI

HIWI (human homolog of drosophila piwi), also called PIWIL1 (piwi-like 1) is an 861 amino acid, 99 kDa (predicted) cytoplasmic protein of the PIWI subgroup of the Argonaute family. PIWI domains bind a class of ~29-30 nucleotide RNAs termed piRNAs (piwi domain-binding RNAs). PIWIL1 orthologs are expressed with piRNAs in germ cells and are essential for spermatogenesis in mice. CD34⁺ hematopoietic progenitor cell PIWIL1 is downregulated upon differentiation. When overexpressed in leukemic cell lines, PIWIL1 inhibits growth and induces apoptosis. Human PIWIL1 shares 95% amino acid sequence identity with mouse and rat PIWIL1.

Long Name

Piwi-like Protein 1

Alternate Names

HIWI, MIWI

Entrez Gene IDs

9271 (Human)

Gene Symbol

PIWIL1

UniProt

NP_004755

Additional PIWIL1/HIWI Products

Product Documents for Human PIWIL1/HIWI Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human PIWIL1/HIWI Antibody

For research use only

Citations
Reviews

Human PIWIL1/HIWI Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6548

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all PIWIL1/HIWI Antibodies »

Product Specifications

Immunogen

Specificity