Human Phospho-LAT (Y161) Alexa Fluor® 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB6334T
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 627945
Product Specifications
Immunogen
Phosphopeptide containing the human LAT Y161 site
Accession # O43561
Accession # O43561
Specificity
Detects human Phospho-LAT (Y161) in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: LAT
LAT (Linker for Activation of T cells) is a type III transmembrane lipid raft protein that serves as a scaffold for signaling molecules. Isoforms of 262 (36 kDa) and 233, called long and short, respectively, differ by inclusion/exclusion of aa 114‑142; a 269 aa isoform lacks this region, but includes alternate N-terminal sequence. Upon T-cell antigen receptor activation, LAT is multiply phosphorylated by ZAP-70/Syk protein tyrosine kinases, creating docking sites for SH2 domain-containing proteins. Phospholipase C-gamma docks at Y161, which is pY132 in the short form of LAT. Mutation of this site results in a Th2 autoimmune lymphoproliferative disorder in mice.
Long Name
Linker for Activation of T cells
Alternate Names
pp36
Gene Symbol
LAT
UniProt
Additional LAT Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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