PEDF R/PNPLA2 was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human PEDF R/ PNPLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5387) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to the sarcolemma of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human PEDFR/PNPLA2 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PEDFR/PNPLA2

PEDF R (Pigment epithelium derived factor receptor; also ATGL, PNPLA2 and desnutrin) is a 55-60 kDa member of the PNPLA family of proteins. It is expressed in adipocytes, hepatocytes, skeletal muscle, and pigment epithelium. PEDF R specifically hydrolyses triglycerides, releasing long-chain fatty acids. Human PEDF R is described as being a 504 amino acid (aa) type II transmembrane (TM) protein. Its TM segment is stated to span aa 9-29. It also contains a palatin domain (aa 10-179) with a conserved GxSxG enzymatic motif. There is one potential isoform variant that shows a deletion of aa 2-62. Over aa 162-332, human PEDF R shares 85% aa identity with mouse PEDF R.

Long Name

Pigment Epithelium-derived Factor Receptor/Patatin-like Phospholipase Domain-containing Protein 2

Alternate Names

ATGL, Desnutrin, IPLA2-zeta, PEDF R, PNPLA2, TTS2

Entrez Gene IDs

57104 (Human); 66853 (Mouse); 361676 (Rat)

Gene Symbol

PNPLA2

UniProt

Q96AD5

Additional PEDFR/PNPLA2 Products

Product Documents for Human PEDFR/PNPLA2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human PEDFR/PNPLA2 Antibody

For research use only

Citations
Reviews

Human PEDFR/PNPLA2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5387

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all PEDFR/PNPLA2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human PEDFR/PNPLA2 Antibody

PEDF R/PNPLA2 in Human Heart.