Human PDCD4 Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7019AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PDCD4
Specificity
Detects human PDCD4 in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human PDCD4 Alexa Fluor™ Plus 488-conjugated Antibody
Application
Recommended Usage
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: PDCD4
PDCD4 (Programmed cell death protein 4; also H731) is a 54-64 kDa member of the PDCD4 family of molecules. It is widely expressed, being found in mammary epithelium, CD34+ bone marrow progenitor cells, fibroblasts and keratinocytes. PDCD4 is both cytoplasmic and nuclear. In the cytoplasm, it blocks protein translation by binding to eIF4A, an act that dissociates eIF4G and mRNA from eIF4A. In the nucleus, it seems to block transcription of select genes, one of which is MAP4K1, a key enzyme in the AP-1-mediated transcription pathway. Human PDCD4 is 469 amino acids (aa) in length. It contains an NLS (aa 58-64), an MI domain (aa 163-284), another NLS (aa 241-250) and a second MI domain (aa 326-449). There are at least seven utilized Ser/Thr phosphorylation sites and one Tyr phosphorylation site. There are two potential splice forms, one that contains a deletion of aa 15-28, and a second that may show a three aa substitution for aa 2-15. Over aa 212-357, human PDCD4 shares 98% aa identity with mouse PDCD4.
Long Name
Programmed Cell Death 4
Alternate Names
H731, Protein 197/15a
Gene Symbol
PDCD4
UniProt
Additional PDCD4 Products
Product Specific Notices for Human PDCD4 Alexa Fluor™ Plus 488-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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