Human Pax7 Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6670N
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Pax7
Met1-Gly196
Accession # P23759
Met1-Gly196
Accession # P23759
Specificity
Detects human Pax7 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Pax3 is observed and approximately 4% cross-reactivity with rhPax1 and rhPax6 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Pax7 Alexa Fluor® 700-conjugated Antibody
Application
Recommended Usage
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Pax7
PAX7 (Paired box 7A; also HuP1) is a 57 kDa member of the paired homeobox family of transcription factors. It is expressed in select neurons (in rodent) and skeletal muscle satellite cells, where, in the latter, it promotes both a muscle stem cell state (PAX7+ desmin- cells) and a lineage commitment state (PAX7+ desmin+ cells). PAX7 forms heterodimers with PAX3, and interacts with the regulatory regions of the Myf5 locus. Human PAX7(A) is 520 amino acids (aa) in length. It contains an N‑terminal paired box DNA-binding domain (aa 34-163), a homeodomain (aa 218-275) and a C-terminal poly-alanine region (aa 340-346). Multiple splice forms of PAX7 exist. There is a 54 kDa isoform B that contains a 38 aa substitution for aa 468-520, plus a second isoform that shows a deletion of Gly151 and Leu152. There is also a chromosomal rearrangement that fuses aa 30-385 of PAX7 to aa 211-265 of FOXO1A/FKHR. Over aa 1-196, human PAX7 shares 86% and 96% aa identity with human PAX3 and mouse PAX7, respectively.
Long Name
Paired Box Gene 7
Alternate Names
HUP1, RMS2
Gene Symbol
PAX7
UniProt
Additional Pax7 Products
Product Documents for Human Pax7 Alexa Fluor® 700-conjugated Antibody
Product Specific Notices for Human Pax7 Alexa Fluor® 700-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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