Human Patched 2/PTCH2 (First Extracellular Loop) Alexa Fluor™ Plus 680-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4078AFP680
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready
Label
Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Patched 2/PTCH2 first extracellular loop
Specificity
Detects human Patched 2/PTCH2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse Patched is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
CyTOF-ready
Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Patched 2/PTCH2
Patched 2 (Ptch2) is a 180-200 kDa 12 transmembrane member of the Patched family. Human PTCH2 is 1203 amino acids (aa) in length. It has two terminal cytoplasmic domains, one between aa 1-57, and a second between aa 1115-1203. Together with Smoothened (SMO), Ptch2 forms a membrane complex that serves as a receptor for Sonic Hedgehog (SHH). Human Ptch2 likely has two isoforms. One shows an eight aa extension of the C-terminus, while a second shows a four aa substitution for the C-terminal 61 amino acids (aa 1143-1203). Over the first extracellular loop human Patched 2 shares 95% and 96% aa sequence identity with mouse and canine Patched 2, respectively.
Alternate Names
ptc2, PTCH2
Gene Symbol
PTCH2
UniProt
Additional Patched 2/PTCH2 Products
Product Specific Notices
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
Recommended applications are based on validated applications from the unconjugated base product AF4078.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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