Human p70 S6 Kinase Alexa Fluor® 405-conjugated Antibody

Name: Human p70 S6 Kinase Alexa Fluor® 405-conjugated Antibody
Brand: R&D Systems
SKU: AF8962V

R&D Systems, part of Bio-Techne | Catalog # AF8962V

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Product Datasheet / COA

Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human p70 S6K Accession # M60725
Applications:	CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Label:	Alexa Fluor 405 (Excitation=405 nm, Emission=421 nm)
Specificity:	Detects human p70 S6K and p85 S6K (also known as p70 S6K alpha I), an isoform with 23 extra residues at the N-terminus. Reactivity with beta isoforms of p70 S6K is unknown.

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Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.
CyTOF-ready	Optimal dilution of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: p70 S6 Kinase

p70 S6 kinase is a Ser/Thr kinase activated by such mitogens as EGF, IGF-I, and insulin. The S6 protein of the 40S ribosomal subunit is a major substrate of p70 S6 kinase, and its phosphorylation upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start site. The activity of p70 S6 kinase is controlled by multiple phosphorylation events, with phosphorylation at T229 by PDK1 and T389 by mTOR most critical for kinase function.

Entrez Gene IDs

6198 (Human); 72508 (Mouse); 83840 (Rat)

Alternate Names

EC 2.7.11, EC 2.7.11.1, P70S6K1, PS6K, RPS6KB1, Ribosomal protein S6 kinase I, S6K, S6K-beta-1, S6K1, S6K1p70-S6K 1, STK14A, Serine/threonine-protein kinase 14A, p70 S6 Kinase, p70 S6 kinase alpha, p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1, p70 S6 kinase, alpha 2, p70 S6K-alpha, p70 S6KA, p70 ribosomal S6 kinase alpha, p70(S6K)-alpha, p70-S6K, p70-alpha, ribosomal protein S6 kinase beta-1, ribosomal protein S6 kinase, 70kD, polypeptide 1, ribosomal protein S6 kinase, 70kDa, polypeptide 1, serine/threonine kinase 14 alpha

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