Human OX40 Ligand/TNFSF4 Antibody

Name: Human OX40 Ligand/TNFSF4 Antibody
Brand: R&D Systems
SKU: MAB10656
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB10656

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived human OX40 Ligand/TNFRSF4 Gln51-Leu183 Accession # Q6FGS4
Applications:	Immunohistochemistry
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 1034083
Specificity:	Detects human OX40 Ligand/TNFRSF4 in direct ELISAs.

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Applications for Human OX40 Ligand/TNFSF4 Antibody

	Recommended Concentration	Sample
Immunohistochemistry	5-25 µg/mL	Immersion fixed paraffin-embedded sections of human testis

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human OX40 Ligand/TNFSF4 Antibody

	Immunohistochemistry OX40 Ligand/TNFRSF4 in Human Testis. OX40 Ligand/TNFRSF4 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human OX40 Ligand/TNFRSF4 Monoclonal Antibody (Catalog # MAB10656) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in sperm cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1¬23) and a carboxy¬terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similarly to other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40 Ligand is one of the co¬stimulatory molecules in the immune system that includes B7, CD40 Ligand, CD30 Ligand, CD27 Ligand and 4¬1BB Ligand. Because OX40 appears as a late activation-induced T cell surface antigen, it has been speculated that the major function of OX40-OX40L interaction is to transmit a late co-stimulatory signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.

Item	Value
References	Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757 Baum, P.R. et al. (1994) EMBO J. 13:3992. AlShamkhani, A. et al. (1997) J. Biol. Chem. 272:5275. Kjaergaard, J. et al. (2000) Cancer Res. 60:5514.
Entrez Gene IDs	7292 (Human); 22164 (Mouse); 89814 (Rat)
Alternate Names	CD134 ligand, CD134L, CD252, CD252 antigen, Glycoprotein Gp34, OX-40L, OX40 Ligand, OX40 antigen ligand, OX40L, OX4OL, TAX transcriptionally-activated glycoprotein 1, TNFSF4, TXGP1, gp34, tax-transcriptionally activated glycoprotein 1 (34kD), tax-transcriptionally activated glycoprotein 1, 34kD, tumor necrosis factor (ligand) superfamily, member 4, tumor necrosis factor ligand superfamily member 4

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Customer Reviews for Human OX40 Ligand/TNFSF4 Antibody (1)

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Human OX40/TNFRSF4 Antibody

Name: Anonymous

Application: IHC

Sample Tested: Testis tissue

Species: Human

Verified Customer | Posted 03/22/2022

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Human OX40 Ligand/TNFSF4 Antibody

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Applications for Human OX40 Ligand/TNFSF4 Antibody

Scientific Data Examples for Human OX40 Ligand/TNFSF4 Antibody

Immunohistochemistry

Formulation, Preparation and Storage

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Background: OX40 Ligand/TNFSF4

Citations for Human OX40 Ligand/TNFSF4 Antibody

Customer Reviews for Human OX40 Ligand/TNFSF4 Antibody (1)

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Human OX40/TNFRSF4 Antibody

Product Documents for Human OX40 Ligand/TNFSF4 Antibody

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