OX40 (CD134; TNFRSF4) is a T cell co-stimulatory molecule of the TNF receptor superfamily that coordinates with other co-stimulators (CD28, CD40, CD30, CD27 and 4-1BB) to manage the activation of the immune response (1-3). Human OX40 is a 48 kDa type I transmembrane glycoprotein with a 28 amino acid (aa) signal sequence, a 185 aa extracellular domain (ECD) that contains a cysteine-rich region, a 20 aa transmembrane segment, and a 41 aa cytoplasmic domain (4). The ECD of human OX40 shares 63% sequence identity with the ECD of mouse and rat OX40. OX40 is up-regulated on CD4⁺ and CD8⁺ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7 (5, 6). OX40 Ligand is primarily expressed on antigen presenting cells (5). OX40 Ligand engagement of OX40 on activated CD4⁺ T cells results in increased T cell survival, proliferation, and cytokine production. It also inhibits the conversion of effector T cells into immunosuppressive regulatory T cells (Tregs) and can promote the maintenance of and recall response in memory T cells (3, 7-10). OX40 is constitutively expressed on Tregs and enhances the sensitivity of Tregs to IL-2, thus promoting Treg proliferation. OX40 has also been shown to decrease the cells’ immunosuppressive activity on effector T cells (11-14). OX40-OX40 Ligand signaling is involved in allergic airway inflammation, graft-versus-host disease and autoimmune disease (6, 15, 16). Mutations in OX40 and OX40 Ligand are associated with cardiovascular disease (17, 18).

References

Hori, T. (2006) Int. J. Hematol. 83:17.
Latza, U. et al. (1994) Eur. J. Immunol. 24:677.
Salek-Ardakani, S. et al. (2003) J. Exp. Med. 198:315.
al-Shamkhani, A. et al. (1996) Eur. J. Immunol. 26:1695.
Moran, A.E. et al. (2013) Curr. Opin. Immunol. 25:230.
Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
Xiao, X. et al. (2008) J. Immunol. 181:3193.
So, T. and M. Croft (2007) J. Immunol. 179:1427.
Mousavi, S.F. et al. (2008) J. Immunol. 181:5990.
Bansal-Pakala, P. et al. (2001) Nat. Med. 7:907.
Piconese, S. et al. (2010) Eur. J. Immunol. 40:2902.
Griseri, T. et al. (2010) J. Exp. Med. 207:699.
Xiao, X. et al. (2012) J. Immunol. 188:892.
Vu, M.D. et al. (2007) Blood 110:2501.
Damayanti, T. et al. (2010) Am. J. Respir. Crit. Care Med. 181:688.
Xiao, X. et al. (2012) Nat. Immunol. 13:981.
Nakano, M. et al. (2010) Cardiovasc. Res. 88:539.
Ishii, N. et al. (2010) Adv. Immunol. 105:63.
Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757

Alternate Names

ACT-135, CD134, Ly-70, TNFRSF4, Txgp1, TXGP1L

Entrez Gene IDs

7293 (Human); 22163 (Mouse); 25572 (Rat); 102145978 (Cynomolgus Monkey)

Gene Symbol

TNFRSF4

UniProt

P43489

Additional OX40 Products

Product Documents

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human OX40 Alexa Fluor™ Plus 488-conjugated Antibody

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human OX40 Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB10545AFP488

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all OX40 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Applications for Human OX40 Alexa Fluor™ Plus 488-conjugated Antibody

CyTOF-ready

Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Shipping

Stability & Storage

Background: OX40