Orexin R2/HCRTR2 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using 25 µg/mL Mouse Anti-Human Orexin R2/HCRTR2 Monoclonal Antibody (Catalog # MAB52461) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Orexin R2/HCRTR2 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Orexin R2/HCRTR2

Orexin R2 (Orexin Receptor 2), also known as HCRTR2 (Hypocretin Receptor 2) or OX2R is a 40 kDa 7-transmembrane G-protein-coupled glycoprotein that is a high affinity receptor for orexins A and B (hypocretins 1 and 2). In mouse brain, engagement of Orexin Rs/HCRTRs promotes wakefulness, such that absence of either orexins or their receptors creates a narcolepsy-like state. It also influences reward circuits involving food or addictive drugs. The extracellular portions of human Orexin R2/HCRTR2 share 92% and 93% aa identity with corresponding portions of mouse and rat Orexin R2/HCRTR2, respectively.

Long Name

Hypocretin Receptor Type 2

Alternate Names

HCRTR2, Orexin Receptor 2, OrexinR2, OX2R

Entrez Gene IDs

3062 (Human); 387285 (Mouse); 25605 (Rat)

Gene Symbol

HCRTR2

UniProt

AAC39602

Additional Orexin R2/HCRTR2 Products

Product Documents for Human Orexin R2/HCRTR2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human Orexin R2/HCRTR2 Antibody

For research use only

Citations
Reviews

Human Orexin R2/HCRTR2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB52461

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Orexin R2/HCRTR2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Orexin R2/HCRTR2 Antibody

Orexin R2/HCRTR2 in Human Brain.