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Human OBCAM/OPCML Antibody

Bio-Techne includes R&D Systems | Catalog # AF2777

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AF2777
AF2777-SP

Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human OBCAM/OPCML Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human OBCAM/OPCML
Gly28-Asn322
Accession # Q14982

Specificity

Detects human OBCAM/OPCML in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) KILON is observed and less than 1% cross-reactivity with rhLAMP is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human OBCAM/OPCML Antibody

OBCAM/OPCML antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

OBCAM/OPCML in MCF‑7 Human Cell Line.

OBCAM/OPCML was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human OBCAM/OPCML Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2777) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human OBCAM/OPCML Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: NG108-15 mouse neuroblastoma/rat glioma hybrid cell line

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed NG108-15 mouse neuroblastoma/rat glioma hybrid cell line and immersion fixed MCF-7 human breast cancer cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Western Blot

0.1 µg/mL
Sample: Recombinant Human OBCAM (Catalog # 2777-CM)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 4 publications using AF2777 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OBCAM/OPCML

OBCAM (Opioid-binding cell adhesion molecule, also known as OPCML) is a member of the IgLON family of cell adhesion molecules. All IgLONs are GPI-linked glycoproteins, contain three C2 type Ig-like domains, and are expressed in cerebral cortex and hippocampus (1, 2). The name IgLON derives from family membership in the Ig superfamily, and the first letters of the names of group’s molecules; LAMP, OBCAM, and Neurotrimin. Recently, membership in the group has been expanded by one with the addition of Kilon (Kindred of IgLON), and members of this group are now often referred to Diglons, based on the dimerizing nature of the IgLONs (1, 2). Human OBCAM is synthesized as a 345 amino acid (aa) preproprecursor that contains a 27 aa signal sequence, a 295 aa mature region, and a C‑terminal 23 aa prosegment (3). The prosegment is cleaved to generate the GPI-link. OBCAM varies in molecular weight, ranging from 46 kDa to 65 kDa (4‑6). The difference is not due to alternate splicing but to differential glycosylation (6). Although it is not unusual for GPI-linked proteins to be solubilized, to date there is no evidence that OBCAM functions as a soluble molecule (1). Mature human OBCAM is 98%, 99%, and 98% aa identical to mature bovine, rat and mouse OBCAM, respectively. OBCAM has limited expression, occurring principally in telencephalon and ovarian epithelium (7, 8). In brain, it is found associated with dendrites and post-synaptic membranes, where it may maintain synaptic architecture (1, 5). In ovary, it has been suggested to be a tumor-suppressor factor (8). The receptor(s) for OBCAM appears to be other members of the IgLON family, and a dimer is the functional unit. While neurotrimin appears to function as both a homodimer and heterodimer, all other family members (including OBCAM) show a preference for heterodimerization. OBCAM forms strong trans (between cells) heterodimers with LAMP, and modest heterodimers with Neurotrimin. There is hardly any binding with itself. Kilon likely binds OBCAM, but this interaction is not well studied (1). OBCAM heterodimers apparently bind to almost all possible IgLON heterodimer combinations on other cells. In cis (same cell), OBCAM also binds to LAMP and Neurotrimin (2).

References

  1. Miyata, S. et al. (2000) Neuroscience 117:645.
  2. Reed, J. et al. (2004) J. Cell Sci. 117:3961.
  3. Shark, K.B. and N.M. Lee (1995) Gene 155:213.
  4. Wick, M.J. et al. (1996) Mol. Brain Res. 36:322.
  5. Miyata, S. (2000) J. Comp. Neurol. 242:74.
  6. Hachisuka, A. et al. (1996) Neurochem. Int. 28:373.
  7. Miyata, S. et al. (2003) Brain Res. 979:129.
  8. Sellar, G.C. et al. (2003) Nat. Genet. 34:337.

Long Name

Opioid-binding Cell Adhesion Molecule/Opioid Binding Protein/Cell Adhesion Molecule-like

Alternate Names

OPCML

Entrez Gene IDs

4978 (Human); 330908 (Mouse)

Gene Symbol

OPCML

UniProt

Additional OBCAM/OPCML Products

Product Documents for Human OBCAM/OPCML Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human OBCAM/OPCML Antibody

For research use only

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