Human NQO-1 Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7567AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NQO-1
Specificity
Detects human NQO-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human NQO-2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human NQO-1 Alexa Fluor™ Plus 488-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: NQO-1
NQO-1 (NADPH Quinone acceptor Oxidoreductase 1; also DTD and Menadione reductase) is a 31-33 kDa cytoplasmic member of the NAD(P)H dehydrogenase family of enzymes. It is widely expressed, being found in adipocytes, endothelial cells and hepatocytes. NQO-1 serves as a cytoprotective molecule, reducing quinones (atmospheric benzene and esrtrogen compounds) and nitroaromatics without consuming sulfhydryl compounds. It also acts as a backup superoxide scavenger to SOD. NQO-1 reportedly binds and stabilizes key cell protection molecules such as p53, thus acting as a gatekeeper for proteosome-mediated protein turnover. Human NQO-1 is 274 amino acids (aa) in length. It contains an FMN reductase domain (aa 5-212) and two utilized Lys acetylation sites. NQO-1 functions as a noncovalent homodimer. There are three potential at Met22, while two others show a deletion of aa 141-174 and 102-139, respectively. Full length human NQO-1 shares 86% aa sequence identity with mouse NQO-1.
Long Name
NAD(P)H Dehydrogenase, Quinone 1
Alternate Names
Azoreductase, DHQU, DIA4, Diaphorase-4, Dioxin-inducible 1, DTD, Menadione Reductase, NMOR1, NQO1, QR1, Quinone Reductase 1
Entrez Gene IDs
1728 (Human)
Gene Symbol
NQO1
UniProt
Additional NQO-1 Products
Product Specific Notices for Human NQO-1 Alexa Fluor™ Plus 488-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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