Human NM23-H1 Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB6256N
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 642817
Product Specifications
Immunogen
E. coli-derived recombinant human NM23‑H1
Met1-Glu152
Accession # P15531
Met1-Glu152
Accession # P15531
Specificity
Detects human NM23‑H1 in Western blots. In Western blots, no cross-reactivity with recombinant human NM23-H2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human NM23-H1 Alexa Fluor® 700-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: NM23-H1
NM23-H1 (Non-metastatic protein 23 homolog 1; also NDKA) is a 19‑20 kDa member of the NDK family of enzymes. NM23-H1 is ubiquitous in expression and performs multiple functions. It forms disulfide-linked homohexamers, and heterohexamers with NM23-H2, generating a nucleoside diphosphate kinase that catalyzes a phosphoryl transfer from ATP to a nucleoside diphosphate. It also shows His and Ser/Thr protein kinase activity and forms covalent linkages with molecules diverse as p53 and STRAP. It is found both intracellularly and in blood at ng/mL concentrations. Human NM23-H1 is 152 amino acids (aa) in length, contains one NDP kinase domain (aa 5‑134), and shows acetylation at Ala2 and Lys56, plus phosphorylation at Tyr52, Thr94, Ser122, and Ser125. Human NM23-H1 shares 89% aa identity with human 17‑18 kDa NM23-H2 and 94% aa identity with mouse NM23-H1. A second H1 isoform named NM23-H1B with 25 additional aa at the N‑terminus has also been described.
Long Name
Non-metastatic Protein 23 Homolog 1
Alternate Names
GAAD, NDKA, NDPKA, NM23A, NM23H1, NME1
Entrez Gene IDs
4830 (Human)
Gene Symbol
NME1
UniProt
Additional NM23-H1 Products
Product Specific Notices for Human NM23-H1 Alexa Fluor® 700-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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