Human NKX3.1 Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6080AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NKX3.1
Specificity
Detects human NKX3.1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) NKX2.2, rhNKX6.1, and rhBAP31 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human NKX3.1 Alexa Fluor™ Plus 488-conjugated Antibody
Application
Recommended Usage
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: NKX3.1
NKX3.1 (Homeobox protein NK-3 homolog A) is a 32-38 kDa member of the NK-3 homeobox family of transcription factors. It is expressed in testis and prostatic epithelium, and appears to be regulated by both testosterone and estrogen (but not progesterone). NKX3.1 apparently promotes prostate gland development, and acts as a tumor suppressor/transcriptional repressor by interacting with HDAC-1 and increasing p53 acetylation and half-life. Human NKX3.1 is 234 amino acids (aa) in length. It contains a DNA binding homeodomain (aa 124-183) and phosphorylation sites at Ser185 and Ser196 that regulate protein turnover. There are four splice variants that show changes N-terminal to the homeobox domain. Deletions of aa 8-56, 13-87, 15-83 and 40-83 are all reported. Over aa 1-123, human NKX3.1 shares 50% aa identity with mouse NKX3.1.
Long Name
NK3 Homeobox 1
Alternate Names
BAPX2, NKX3-1, NKX3A
Gene Symbol
NKX3-1
UniProt
Additional NKX3.1 Products
Product Specific Notices for Human NKX3.1 Alexa Fluor™ Plus 488-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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