Human NG2/MCSP Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2585AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Knockout Validated, Western Blot
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Specificity
Detects human NG2/MCSP in direct ELISAs and Western blots. In Western blots, approximately 30% cross-reactivity with recombinant mouse CSPG4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Knockout Validated
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: NG2/MCSP
NG2 (neuron/glia-type 2 antigen; also MCSP/melanoma chondroitin sulfate proteoglycan) is a 250‑500 kDa type I integral membrane proteoglycan found on synantocytes (NG2+ glia), vascular pericytes, chondroblasts, macrophages and melanoma cells. It binds multiple components of the ECM, and serves as a ligand for alpha3 beta1 and alpha4 beta1 integrins. Mature human NG2 is 2293 amino acids (aa) in length. It contains a large 2195 aa extracellular region (aa 30‑2224) and a short 79 aa cytoplasmic tail. NG2 is variably glycanated. Without proteoglycan, NG2 is expressed as a 250 kDa glycoprotein. Juxtamembrane proteolysis generates a soluble ECD that noncovalently associates with the transmembrane fragment. Over aa 1583‑2224, human NG2 is 81% aa identical to both mouse and canine NG2.
Long Name
Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
Alternate Names
AN2, CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG
Gene Symbol
CSPG4
UniProt
Additional NG2/MCSP Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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