Human Neutrophil Elastase/ELA2 Alexa Fluor® 700-conjugated Antibody

Name: Human Neutrophil Elastase/ELA2 Alexa Fluor® 700-conjugated Antibody
Brand: R&D Systems
SKU: FAB91672N

R&D Systems, part of Bio-Techne | Catalog # FAB91672N

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Product Datasheet / COA

Key Product Details

Species Reactivity:	Human
Immunogen:	Chinese hamster ovary cell line CHO-derived recombinant human Neutrophil Elastase/ELA2 Met1-Asn252 Accession # P08246
Applications:	ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label:	Alexa Fluor 700 (Excitation=675-700 nm, Emission=723 nm)
Specificity:	Detects human Neutrophil Elastase/ELA2 in direct ELISAs.

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Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
ELISA	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.
Immunocytochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: Neutrophil Elastase/ELA2

Neutrophil Elastase (ELA2, ELANE), also known as HNE, is a chymotrypsin family serine protease that plays a key role in pathogen clearance (1-3). It is expressed by promyelocytes and stored in the intracellular azurophilic granules of polymorphonuclear leukocytes (PMN) (4). These granules fuse with phagosomes, enabling Neutrophil Elastase to participate in the digestion and killing of endocytosed microbes. The enzyme is released by activated neutrophils at sites of inflammation, and it can remain associated with the cell surface or function as a component of neutrophil extracellular nets (NETs) which trap and kill microbial pathogens (5, 6). It also can degrade multiple extracellular matrix proteins including Elastin and Fibronectin (5). In the lung, this activity contributes to pathology in emphysema, cystic fibrosis, and adult respiratory distress syndrome (ARDS) (1). Neutrophil Elastase can be inhibited by Serpin A1/alpha 1-Antitrypsin, SLPI, Serpin B1, and
Trappin-2/Elafin (7-11). Its activity in the lung is increased by exposure to tobacco smoke which inactivates Serpin A1 through methionine oxidation (12). Mature human Neutrophil Elastase shares 73% amino acid sequence identity with mouse and rat Neutrophil Elastase (13, 14). Multiple mutations in the human ELANE gene are causative of severe congenital and cyclic neutropenias (15).

Item	Value
Entrez Gene IDs	1991 (Human); 50701 (Mouse)
Alternate Names	Bone marrow serine protease, EC 3.4.21, EC 3.4.21.37, ELA2, ELA2granulocyte-derived elastase, ELANE, Elastase-2, GE, HLEelastase-2, HNE, Human leukocyte elastase, Leukocyte Elastase, Medullasin, NE, Neutrophil Elastase, PMN elastase, PMN-E, SCN1, elastase 2, neutrophil, elastase, neutrophil expressed, polymorphonuclear elastase

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