Detects human Neprilysin‑2/MMEL1 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Neprilysin is observed and less than 1% cross-reactivity with rhECE-1 and rhECE-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Neprilysin-2/MMEL1 Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Neprilysin-2/MMEL1 (Catalog # 2340-ZN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neprilysin-2/MMEL1

Neprilysin-2 is a zinc protease of the neprilysin (NEP) family, which also includes NEP, ECE-2, PEX, DINE, Kell and several NEP-like proteins (1). It is encoded by the MMEL1 gene in the human genome and is also known as soluble secreted endopeptidase (SEP), Neprilysin-like 1 and Neprilysin-2 (2). Highly expressed in testis, the cDNA predicted a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Both membrane-bound and soluble forms were comparable with regard to model substrates, pH optima and inhibitor profiles. The ectodomain of human Neprilysin-2/MMEL1 was expressed with an N-terminal His tag and purified.

References

Turner, A.J. et al. (2001) BioEssays 23:261.
Carpentier, M. et al. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. p. 426.

Alternate Names

MMEL1, MMEL2, Neprilysin2, NL2, SEP

Entrez Gene IDs

79258 (Human)

Gene Symbol

MMEL1

UniProt

Q495T6

Additional Neprilysin-2/MMEL1 Products

Product Documents for Human Neprilysin-2/MMEL1 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human Neprilysin-2/MMEL1 Biotinylated Antibody

For research use only

Citations
Reviews

Human Neprilysin-2/MMEL1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF2340

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity