Human NCOA2 Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF8010AFP488

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AF8010AFP488-100UG
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Sheep IgG

View all NCOA2 Antibodies »

Product Specifications

Specificity

Detects human NCOA2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) NCOA1 is observed, and less than 1% cross-reactivity with rhNCOA4 and rhNCOA6 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human NCOA2 Alexa Fluor™ Plus 488-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Background: NCOA2

NCOA2 (Nuclear receptor COActivator 2; also TIF2 and GRIP-1) is a 150-160 kDa, ubiquitously expressed, nuclear member of the SRC/p160 nuclear receptor (NR) coactivator family of proteins. As noted, and although NCOA2 is a coactivator of hormone nuclear receptors, it also contributes to HIF-1 alpha:Arnt driven gene transcription. With respect to NRs, it not only has intrinsic activity that directly impacts hormone receptor gene activation, but also recruits additional coactivators to the NR:p160/NCOA2 complex, imparting additional complexity to target gene expression. NCOA2 binds directly to nuclear receptors via one or more LxxLL motifs that constitute an NID (NR Interaction Domain). It further uses its C-terminus to both recruit additional coactivators such as CBP and CARM1, and to modify key chromatin sites via an intrinsic HAT activity. Human NCOA2 is 1464 amino acids (aa) in length. It contains bHLH domain (aa 26-83), a PAS region (aa 119-183), four consecutive LxxLL motifs over aa 641-882, and a C-terminal LLxxLxxxL segment (aa 1079-1087). There are five utilized Ser/Thr phosphorylation sites, and three utilized acetylation sites on Lys. NCOA2 is known to undergo translocation and fuse with KAT6A/MOZ, creating a fusion product containing aa 870-1464 of NCOA2. Over aa 1090-1247, human NCOA2 shares 94% aa sequence identity with mouse NCOA2/GRIP-1.

Long Name

Nuclear Receptor Co-activator 2

Alternate Names

bHLHe75, HTIF2, KAT13C, TIF2

Entrez Gene IDs

10499 (Human); 17978 (Mouse); 83724 (Rat)

Gene Symbol

NCOA2

UniProt

Q15596 (Human)

Additional NCOA2 Products

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Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human NCOA2 Alexa Fluor™ Plus 488-conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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