Human MyBPC3 Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7439S
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MyBPC3
Arg1001-Lys1103
Accession # Q14896
Arg1001-Lys1103
Accession # Q14896
Specificity
Detects human MyBPC3 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse MyBPC3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human MyBPC3 Alexa Fluor® 750-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: MyBPC3
MyBPC3 (MYosin Binding Protein C3; also cardiac MyBP-C, and C-protein, cardiac muscle isoform) is a 150-165 kDa member of the MyBP family, immunoglobulin superfamily of molecules. MyBP-C is expressed in striated muscle and exists in three isoforms; C1, C2, and C3. C1 and C2 are associated with slow and fast skeletal muscle, and may coexist in the same myofiber. The C3 isoform is cardiac specific, and is found in the two dark C-zones that flank the M line of the sarcomere A-band. The A-band contains both actin and myosin, and MyBPC3 forms structures that cross-link the resident actin and myosin filaments. MyBPC3 is reported to interact with titin and light meromyosin with its C-terminus, and actin plus myosin heavy chain with its N-terminus. Human MyBPC3 is 1273 amino acids (aa) in length. It contains one N-terminal I-set Ig-like domain (aa 12-94), a C2-type Ig-like domain (aa 153-256) followed by a phosphorylated myosin-binding region (aa 258-352), four C2-type Ig-like domains (aa 362-770) and three FN type III repeats interspersed with two C2-type Ig-like domains (aa 771-1273). There is one potential alternative start site at Met348. Over aa 1001-1103, human MyBPC3 shares 89% aa identity with mouse MyBPC3.
Long Name
Myosin Binding Protein C, Cardiac
Alternate Names
C-protein, cardiac muscle isoform, CMH4, FHC, MYBP-C
Gene Symbol
MYBPC3
UniProt
Additional MyBPC3 Products
Product Specific Notices for Human MyBPC3 Alexa Fluor® 750-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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