Human MuSK Antibody

R&D Systems, Inc. a Bio-Techne Brand

Human MuSK Antibody
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AF3904-SP
AF3904
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Key Product Details

Species Reactivity: Human
Immunogen: Mouse myeloma cell line NS0-derived recombinant human MuSK
Glu22-Ser503
Accession # O15146
Applications: Western Blot
Label: Unconjugated
Source: Polyclonal Goat IgG
Specificity:

Detects human MuSK in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant rat MuSK is observed.
  • Preparation & Storage

    • Formulation, Preparation and Storage

    Purification Antigen Affinity-purified
    Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.

    Reconstitution Buffer Available:
    Size / Price
    Qty
    Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
    Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
    Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
  • Citations
  • Reviews

    • Citations for Human MuSK Antibody (2)

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    Customer Reviews for Human MuSK Antibody (2)

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    • Human MuSK Antibody
      Name: Mia Huang
      Application: Simple Western
      Sample Tested: Differentiated C2C12 myoblast cells
      Species: Mouse
      Verified Customer | Posted 05/19/2017
      C2C12 differentiated myotubes, following IP with an anti-MuSK antibody (different from this Ab), SDS-PAGE, and transferred into PVDF membrane. After blocking with 5% BSA/TBST, blot was probed with 1:400 of AF562 (4C, o/n). Subsequent probing with a HRP-anti-goat secondary Ab yielded excellent publishable results, with 100 kDa MuSK band visible.
      Human MuSK Antibody Simple Western AF3904
    • Human MuSK Antibody
      Name: Anonymous
      Application: WB
      Sample Tested: Differentiated C2C12 myoblast cells
      Species: Mouse
      Verified Customer | Posted 05/16/2017
      Differentiated C2C12 mouse skeletal muscle lysates IP'ed for MuSK using a donated Ab. IP works well and I can detect phosphorylation of MuSK using a general phosphotyrosine Ab, but upon stripping, blocking, and probing with this Ab, I am unable to detect MuSK (perhaps due to high background signal). Probing with a different MuSK Ab is much cleanerTechnical Service is following up
      Human MuSK Antibody Western Blot AF3904

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    Product Documents for Human MuSK Antibody

    Certificate of Analysis

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