MSX1 was detected in immersion fixed paraffin-embedded sections of human ovary using Mouse Anti-Human MSX1 Monoclonal Antibody (Catalog # MAB5045) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human MSX1 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovary

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSX1

MSX1 (Msh homeobox homology 1) is a member of the muscle segment homoebox gene family. MSX1 is involved in limb-pattern formation, craniofacial development, odontogenesis, and tumor growth inhibition. MSX1 functions as a transcriptional repressor. MSX1 has been shown to interact with the linker histone, H1B, and repress transcription of the MyoD promoter. Chromosomal abnormalities involving MSX1 have been associated with the Wolf-Hirschhorn syndrome characterized by heart defects and mental retardation.

Long Name

Msh Homeobox 1

Alternate Names

HOX7, HYD1, OFC5

Entrez Gene IDs

4487 (Human); 17701 (Mouse); 81710 (Rat)

Gene Symbol

MSX1

UniProt

P28360

Additional MSX1 Products

Product Documents for Human MSX1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MSX1 Antibody

For research use only

Citations
Reviews

Human MSX1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5045

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all MSX1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human MSX1 Antibody

MSX1 in Human Ovary.