Human/Mouse ZAG Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB47641
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 842014
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ZAG
Met1-Ser298
Accession # P25311
Met1-Ser298
Accession # P25311
Specificity
Detects human ZAG in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse ZAG Antibody
ZAG in MCF‑7 Human Cell Line.
ZAG was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human ZAG Monoclonal Antibody (Catalog # MAB47641) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
ZAG in 3T3‑L1 Mouse Cell Line.
ZAG was detected in immersion fixed 3T3-L1 mouse embryonic fibroblast adipose-like cell line using Mouse Anti-Human ZAG Monoclonal Antibody (Catalog # MAB47641) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse ZAG Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line and 3T3-L1 mouse embryonic fibroblast adipose-like cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line and 3T3-L1 mouse embryonic fibroblast adipose-like cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ZAG
ZAG (zinc-alpha 2-glycoprotein; also ZA2G) is a 40 kDa, secreted member of the MHC class I family of proteins. It is produced by adipocytes and various epithelial cells that generate exocrine-type secretions. ZAG is reported to stimulate lipid breakdown and thus may play a role in lipid homeostasis. Mature human ZAG is 278 amino acids (aa) in length. It contains one MHC class I antigen region (aa 26‑201) and a C2‑type Ig‑like domain (aa 207‑292). Two alternate splice forms exist; one shows a 66 aa substitution for the C‑terminal 30 aa, and a second shows a nine Lys substitution for aa 151‑298. Mature human ZAG is 60% aa identical to mouse ZAG.
Long Name
Zinc alpha-2-Glycoprotein
Alternate Names
AZGP1, ZA2G, ZNGP1
Gene Symbol
AZGP1
UniProt
Additional ZAG Products
Product Documents for Human/Mouse ZAG Antibody
Product Specific Notices for Human/Mouse ZAG Antibody
For research use only
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