Human/Mouse SPRY2 Alexa Fluor™ Plus 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6157AFP405
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SPRY2
Specificity
Detects human and mouse SPRY2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: SPRY2
SPRY2 (sprouty homolog 2) is a 35 kDa member of the sprouty family of proteins. It is widely expressed in embryonic and adult tissues, including endothelium. SPRY2 is considered a negative regulator of Ras/ERK signaling. For example, it appears to form a trimeric complex with PKC delta and PKD1, blocking ERK phosphorylation. It also interacts with a number of other molecules, including CBL and SIAH2, which likely limit its availability. Human SPRY2 is 315 amino acids (aa) in length and contains one CBL-TKB binding site (aa 53‑59) that is phosphorylated at Tyr55, a Ser-rich region (aa 125‑131), a Cys-rich domain (aa 178‑301) and a PxxPxR motif that mediates ERK inhibitory activity. Over aa 1‑175, human SPRY2 shares 94% aa identity with mouse SPRY2.
Long Name
Sprouty Homolog 2
Alternate Names
sprouty 2
Gene Symbol
SPRY2
UniProt
Additional SPRY2 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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