Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC3765A
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 527327
Product Specifications
Immunogen
E. coli-derived recombinant human RUNX3/CBFA3
Lys186-Tyr415
Accession # Q13761
Lys186-Tyr415
Accession # Q13761
Specificity
Detects human and mouse RUNX3/CBFA3 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody
Detection of RUNX3/CBFA3 in Human PBMC lymphocytes by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMC) lymphocytes either (A) untreated or (B) treated with PMA were stained with Mouse Anti-Human/Mouse RUNX3/CBFA3 APC-conjugated Monoclonal Antibody (Catalog # IC3765A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMC) lymphocytes treated with PMA were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human peripheral blood mononuclear cells (PBMC) lymphocytes treated with PMA were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: RUNX3/CBFA3
RUNX3, also known as CBFA3, AML-2 or PEBP2-alpha C, is a member of the Runt domain family of nuclear transcriptional regulators. All of the RUNX proteins form dimers with CBF-beta. The Runt domain, amino acids (aa) 54-186, is required for DNA binding, while a pro/ser/thr-rich region (aa 191-415) transcriptionally activates target genes. Isoform 2 has an alternate 19 aa in place of the N-terminal 5 aa of isoform 1. The 415 aa Human RUNX3 shares 91% aa identity with mouse or rat RUNX3. RUNX3 is necessary for growth control of gastric epithelium, neurogenesis of dorsal root ganglia, and T cell differentiation. RUNX3 expression is frequently mutated in tumors and appears to be silenced by methylation.
Long Name
Runt-related Transcription Factor 3
Alternate Names
AML2, CBFA3, PEBP2A3, PEBP2AC
Gene Symbol
RUNX3
UniProt
Additional RUNX3/CBFA3 Products
Product Documents for Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody
Product Specific Notices for Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody
For research use only
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