Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Human SMC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3294) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SMC1 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat SMC1 Antibody

Application

Recommended Usage

Western Blot

0.5 µg/mL
Sample: NRK rat normal kidney cell, HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMC1

Structural Maintenance of Chromosome 1 (SMC1) is an ATPase which, with SMC3, forms a heterodimeric complex. These dimers form a V-shaped molecule with two long coiled-coil arms, each having an ATP-binding domain at its distal end. The SMC1/SMC3 heterodimer plays an important role in sister chromatid cohesion during metaphase, associates with the nuclear matrix and centrosome during interphase and localizes to kinetochores, spindles and spindle poles during mitosis. Smc1 is also a component of the DNA damage response and participates in the cellular response to DNA damage through its phosphorylation on Ser 957 and 966.

Long Name

Structural Maintenance of Chromosomes 1

Alternate Names

SB1.8, SMC1A, SMC1L1

Entrez Gene IDs

8243 (Human); 63996 (Rat)

Gene Symbol

SMC1A

UniProt

Q14683.2

Additional SMC1 Products

Product Documents for Human/Mouse/Rat SMC1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat SMC1 Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat SMC1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3294

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all SMC1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat SMC1 Antibody

Detection of Human SMC1 by Western Blot.