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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 725001

Product Specifications

Immunogen

E. coli-derived recombinant human SHC1
Pro379-Val470, Trp488-Val579
Accession # P29353

Specificity

Detects human SHC1 in direct ELISAs and human, mouse, and rat SHC1 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human SHC3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human/Mouse/Rat SHC1 Alexa Fluor® 532-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SHC1

SH2 domain-containing transforming protein C1 (SHC1), also known as ShcA, is a cytoplasmic adaptor protein that is important in the signal transduction from growth factor, cytokine, and lymphocyte antigen receptors. SHC1 contains a PTB/PID domain (aa 156-339), a collagen homology domain (aa 340-487), and an SH2 domain (aa 488-579). Alternate splicing generates additional isoforms that differ in the extent of N-terminal truncation. Activation of SHC1 by phosphorylation at Ser239, Ser240, and Tyr317 enables Shc1 to interact with the GRB2/SOS complex, leading to the transcription of genes involved in mitogenesis and apoptosis. The p46 and p52 isoforms promote mitogenic signaling, while the p66 isoform promotes apoptosis and functions as a negative regulator of SHC1-mediated mitogenic signal transduction. Within aa 488-579, human SHC1 shares 100% aa sequence identity with mouse and rat SHC1.

Long Name

SHC-transforming Protein 1

Alternate Names

SHCA

Entrez Gene IDs

6464 (Human); 20416 (Mouse); 85385 (Rat)

Gene Symbol

SHC1

UniProt

Additional SHC1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat SHC1 Alexa Fluor® 532-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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