Human/Mouse/Rat RBBP4 Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB7416N
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 968701
Product Specifications
Immunogen
E. coli-derived recombinant human RBBP-4
Ala2-Ser425
Accession # 09028
Ala2-Ser425
Accession # 09028
Specificity
Detects human RBBP-4 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human/Mouse/Rat RBBP4 Alexa Fluor® 700-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: RBBP4
RBBP4 (Retinoblastoma-Binding Protein 4; also RbAp48 and CAF-1) is a 48-56 kDa member of the WD Repeat RBAP46/48/MSI1 family of proteins. It is ubiquitously expressed, and acts as a transcriptional repressor. At the start of G1 of the cell cycle, Rb (retinoblastoma protein) normally associates with an E2F transcription complex on E2F responsive genes, blocking E2F activity. At the appropriate time, Rb is phosphorylated, causing its dissociation from E2F and resulting in E2F activation. Dephosphorylated Rb apparently mediates transcriptional repression by recruiting a histone deacetylase (HDAC), followed by HDAC binding to RBBP4. RBBP4, being a histone-binding protein, now brings histones plus HDACs together, resulting in histone deacetylation and gene silencing. Human RBBP4 is 425 amino acids (aa) in length. It contains six WD repeats (aa 122-403), two N-terminal acetylation sites, and two serine phosphorylation sites at positions 110 and 146. There are at least two isoform variants. One contains a six aa substitution for aa 405-425, while another utilizes an alternative start site at Met36. Human and mouse RBBP4 are identical in aa sequence.
Long Name
Retinoblastoma Binding Protein 4
Alternate Names
CAF-1 p48, CAF-1C, NURF55, RBAP48
Gene Symbol
RBBP4
UniProt
Additional RBBP4 Products
Product Specific Notices for Human/Mouse/Rat RBBP4 Alexa Fluor® 700-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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