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Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody

Bio-Techne includes R&D Systems | Catalog # AF2998

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AF2998
AF2998-SP

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody

Immunogen

E. coli-derived recombinant human PI 3-Kinase p85 alpha
Leu328-Tyr431
Accession # P27986

Specificity

Detects endogenous human, mouse and rat PI 3-Kinase p85 alpha in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody

Detection of Human/Mouse/Rat PI 3-Kinase p85 a antibody by Western Blot.

Detection of Human/Mouse/Rat PI 3-Kinase p85 alpha by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PI 3‑Kinase p85 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2998) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PI 3‑Kinase p85 alpha at approximately 85 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, mouse brain tissue, and rat brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using AF2998 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PI 3-Kinase p85 alpha

Class I phosphoinositide 3-kinases (PI 3-Kinases) are heterodimeric proteins that consist of a catalytic subunit of 110‑120 kDa and an associated regulatory subunit. p85 alpha is one of the regulatory subunits that associate with the class IA PI 3-Kinases. Composed of one SH3 and two SH2 domains, p85 alpha functions as an adapter, coupling catalytic p110 to activated receptor tyrosine kinases.

Long Name

PI 3-Kinase p85 alpha

Alternate Names

GRB1, PI 3Kinase p85 alpha, PIK3R1

Entrez Gene IDs

5295 (Human)

Gene Symbol

PIK3R1

UniProt

Additional PI 3-Kinase p85 alpha Products

Product Documents for Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody

For research use only

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