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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 668619

Product Specifications

Immunogen

E. coli-derived recombinant human p55 γ
Lys251-Gly378
Accession # Q92569

Specificity

Detects human, mouse, and rat PI 3‑Kinase p55 gamma in Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) PIK3R1 (aa 519‑644) and no cross-reactivity with rhPIK3R1 (aa 328-431), rhPIK3R2 (aa 325-428), rhPIK3R2 (aa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PI 3-Kinase p55 gamma

p55 gamma (Protein of 55 kDa, gamma subunit; also PI3-kinase regulatory subunit gamma and p55PIK) is a 55-57 kDa member of the PI3K p85 family of regulatory subunits. It is widely expressed, and serves as a recruitment subunit for the p110 catalytic subunit of PI3 kinase. p55 gamma/p55PIK should not be confused with the 55 kDa alpha splice variant of the p85a gene (75% amino acid [aa] identity). Human p55 gamma is 461 aa in length. It contains a unique 34 aa N-terminus that binds Rb and prevents cycle progression, followed by a Pro-rich region (aa 35-44) and two SH2 domains that bind the p110 catalytic subunit (aa 65-160 and 358-452). Based on mouse, it is likely that alternate start sites at Met8 and Met32 generate 54 and 50 kDa protein products, respectively. There is also a potential for a start site 18 aa upstream of the standard start site, as well as a splice form that shows a deletion of aa 256-314 that may be accompanied by an additional deletion of aa 36-71. Over aa 251-378, human p55 gamma shares 94% aa identity with mouse p55 gamma.

Long Name

PI 3-Kinase p55 gamma

Alternate Names

p55PIK, PI 3Kinase p55 gamma, PIK3R3

Entrez Gene IDs

8503 (Human); 18710 (Mouse); 60664 (Rat)

Gene Symbol

PIK3R3

UniProt

Additional PI 3-Kinase p55 gamma Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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