Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Alexa Fluor® 700-conjugated Antibody

Name: Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Alexa Fluor® 700-conjugated Antibody
Brand: R&D Systems
SKU: AF892N

R&D Systems, part of Bio-Techne | Catalog # AF892N

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Product Datasheet / COA

Key Product Details

Species Reactivity:	Human, Mouse, Rat
Immunogen:	Phosphopeptide containing human RSK1 S221 site
Applications:	Immunohistochemistry, Western Blot
Label:	Alexa Fluor 700 (Excitation=675-700 nm, Emission=723 nm)
Specificity:	Detects human, mouse, and rat RSK1 when phosphorylated at S221, and human and mouse RSK2 when phosphorylated at S227.

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Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: RSK1

The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSKs are directly phosphorylated by ERK1 and ERK2, leading to RSK autophosphorylation and activation. Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB.

Item	Value
Long Name	Ribosomal Protein S6 Kinase I
Entrez Gene IDs	6195 (Human); 20111 (Mouse); 81771 (Rat)
Alternate Names	90kD, polypeptide 1), EC 2.7.11, EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1, HU-1, MAP kinase-activated protein kinase 1a, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, MAPKAPL1A, RPS6KA1, RSK, RSK-1, RSK1, RSK1p90-RSK 1, Ribosomal S6 kinase 1, S6K-alpha 1, S6K-alpha-1, p90RSK1, p90S6K, ribosomal protein S6 kinase alpha 1, ribosomal protein S6 kinase alpha-1, ribosomal protein S6 kinase, 90kD, polypeptide 1, ribosomal protein S6 kinase, 90kDa, polypeptide 1

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