Human/Mouse/Rat Phospho-CREB (S133) Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2510

Discontinued Product
AF2510 has been discontinued. View all CREB products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all CREB Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human, mouse, and rat CREB S133 site

Specificity

Detects human, mouse, and rat CREB when phosphorylated at S133 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Phospho-CREB (S133) Antibody

Detection of Human Phospho-CREB (S133) antibody by Western Blot.

Detection of Human Phospho-CREB (S133) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-CREB (S133) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2510), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-CREB (S133) at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Phospho-CREB (S133) antibody by Simple WesternTM.

Detection of Human Phospho-CREB (S133) by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-CREB (S133) at approximately 54 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-CREB (S133) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2510). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat Phospho-CREB (S133) Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with PMA

Western Blot

0.5 µg/mL
Sample: PMA-treated HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CREB

The cAMP response element binding protein (CREB) belongs to the bZIP superfamily of transcription factors, containing a basic domain that mediates DNA binding and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA. When phosphorylated at Ser133, CREB also binds the coactivator CREB binding protein (CBP), which enhances transcription by acetylating histones to facilitate chromatin unraveling.

Long Name

cAMP Response Element-binding Protein

Alternate Names

CREB1

Entrez Gene IDs

1385 (Human); 12912 (Mouse)

Gene Symbol

CREB1

Additional CREB Products

Product Documents for Human/Mouse/Rat Phospho-CREB (S133) Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Phospho-CREB (S133) Antibody

For research use only

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