Human/Mouse/Rat Netrin-1 Alexa Fluor® 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6419T
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Netrin-1
Val22-Ala604
Accession # O95631
Val22-Ala604
Accession # O95631
Specificity
Detects human, mouse, and rat Netrin-1 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Netrin-1
Netrin-1 (from Sanskrit netr meaning "to guide"; also known as Epididymis tissue protein Li 131P) is a 66-76 kDa secreted member of the laminin superfamily of molecules. It is expressed by multiple cell types, including embryonic neural tube ventral midline cells plus postnatal renal tubule epithelium and oligodendroglia. Netrin-1 has multiple receptors, including beta4-integrins, DCC, DSCAM and UNC5A-thru-D. Its effects are context specific. For example, on neurons, binding to either DCC or DSCAM alone promotes chemoattraction, while a DCC:UNC5 complex promotes chemorepulsion. In addition, the ligation of UNC5B on leukocytes suppresses cytokine production, while the binding of netrin to DCC expressed on select cell types inhibits apoptosis. Mature human netrin-1 is 580 amino acids (aa) in length (aa 25-604). It contains an N-terminal laminin domain (aa 47-284), three EGF-like domains (aa 285-453) and a C-terminal cell-interaction netrin-like domain (aa 472-601). Over aa 22-604, human netrin-1 shares 99% aa sequence identity with mouse netrin-1.
Alternate Names
Netrin1, NTN1
Gene Symbol
NTN1
UniProt
Additional Netrin-1 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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