Human/Mouse/Rat NEDP1/SENP8 Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7760N
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NEDP1/SENP8
Met1-Lys212 (Thr207Ala)
Accession # Q96LD8
Met1-Lys212 (Thr207Ala)
Accession # Q96LD8
Specificity
Detects human, mouse, rat NEDP1/SENP8 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: NEDP1/SENP8
NEDP1 (NEDD8-specific protease 1; also DEN1/Deneddylase-1 Protease, cysteine 2 and SENP8/Sentrin-specific protease 8) is a 24-26 kDa cytoplasmic cysteine protease that belongs to the Ulp/peptidase C48 family of enzymes. It is widely expressed, and is involved in NEDD8-associated ubiquitination. NEDD8 is a 9 kDa polypeptide that covalently binds to, and activates cullin-1, a molecule associated with a ubiquitin ligase complex. NEDD8-activated cullin promotes the transfer of ubiquitin to a limited number of target substrates involved in the cell cycle. This has the effect of controlling the levels of cell cycle regulators within the cell. NEDP1 influences these activities in two ways; first, it converts preNEDD8 into its mature form, and second, it deconjugates NEDD8 from its bound substrates. Human NEDP1 is 212 amino acids (aa) in length. It possesses an acetylated initiating methionine plus a protease domain that spans aa 11-174. Over an equivalent sequence, full-length human NEDP1 shares 92% aa sequence identity with mouse NEDP1. The mouse NEDP1 molecule contains a nine aa extension at the C-terminus not shared by the human molecule.
Alternate Names
DEN1, Deneddylase-1, PRSC2, SENP8
Gene Symbol
SENP8
UniProt
Additional NEDP1/SENP8 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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