Human/Mouse/Rat NEDD8 Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4936AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Specificity
Detects human, mouse, and rat NEDD8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: NEDD8
NEDD8 (Neural precursor cell-expressed developmentally down-regulated gene 8; also Rub1) is a 6-8 kDa member of the ubiquitin family of proteins. It is expressed in striated muscle, and via its C-terminal Gly, forms covalent bonds with cullin family proteins. This conjugation generates ubiquitin ligase activity that regulates cell cycle proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. It contains one ubiquitin-like domain (aa 1-65), and a 4-5 aa C-terminal propeptide that is cleaved to expose a C-terminal Gly residue that is used to generate a Gly-Lys intermolecular bond. Mature human NEDD8 shows 100% aa sequence identity to NEDD8 from both mouse and canine.
Long Name
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8
Alternate Names
Neddylin, Rub1
Gene Symbol
NEDD8
UniProt
Additional NEDD8 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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