Detects endogenous human, mouse and rat MST2 in Western blots. In Western blots, this antibody shows no cross‑reactivity with recombinant human MST1/STK4.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat MST2/STK3 Antibody

Detection of Human/Mouse/Rat MST2/STK3 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat MST2/STK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4549) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MST2/STK3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse/Rat MST2/STK3 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MST2/STK3

MST2 (mammalian sterile twenty-like 2; also known as STK3 and Krs-1) is a 58 kDa member of the GCKII group of the STE20 subfamily of the STE Ser/Thr kinase protein family. In human, MST2 is 491 amino acids (aa) in length. It contains one kinase domain (aa 27‑278), two NESs (aa 361‑371 and 438‑447) and coiled-coil domains (aa 287‑328 and 442‑475), and one NLS (aa 473‑487). A caspase-cleavage site exists between Asp322‑Ser323. MST2 is normally inactive in the cytoplasm bound to Raf-1. Cell activation induces Raf-1:MST2 dissociation, MST2 cleavage, and N-terminal translocation to the nucleus. Human MST2 is 99% and 96% aa identical to canine and mouse MST2, respectively.

Alternate Names

KRS1, Mess1, MST3, STK3

Entrez Gene IDs

6788 (Human); 56274 (Mouse)

Gene Symbol

STK3

UniProt

Q13188

Additional MST2/STK3 Products

Product Documents for Human/Mouse/Rat MST2/STK3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat MST2/STK3 Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat MST2/STK3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4549

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all MST2/STK3 Antibodies »

Product Specifications

Immunogen

Specificity