Human/Mouse/Rat MKK6 Alexa Fluor™ Plus 680-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF16041AFP680
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Western Blot
Label
Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MKK6
Specificity
Detects human, mouse, and rat MKK6.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Knockout Validated
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: MKK6
Mitogen-activated protein kinase kinase 6 (MKK6), also known as MEK6, and the closely related MKK3 are activated by pro-inflammatory cytokines and environmental stresses (1, 2). Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr (3). Activation of MKK6 occurs through phosphorylation at Ser207 and Thr211 (4) by several upstream MAPK kinase kinases, including ASK1 and TAK1 (5, 6). Purification of E. coli-expressed wild-type active MKK6 capable of p38 alpha phosphorylation has been previously noted (7).
References
- Goedert, M. et al. (1997) EMBO J. 16:3563.
- Moriguchi, T. et al. (1996) J. Biol. Chem. 271:26981.
- Raingeaud, J. et al. (1995) J. Biol. Chem. 270:7420.
- Han, J. et al. (1996) J. Biol. Chem. 271:2886.
- Ichijo, H. et al. (1997) Science 275:90.
- Moriguchi, T. et al. (1996) J. Biol. Chem. 271:13675.
- Keesler, G. et al. (1998) Protein Expr. Purif. 14:221.
Long Name
Mitogen-activated Protein Kinase Kinase 6
Alternate Names
MAP2K6, MEK6, PRKMK6, SAPKK3
Gene Symbol
MAP2K6
UniProt
Additional MKK6 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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