Human/Mouse/Rat MIF Alexa Fluor® 647-conjugated Antibody

Name: Human/Mouse/Rat MIF Alexa Fluor® 647-conjugated Antibody
Brand: R&D Systems
SKU: AF-289-PBR

R&D Systems, part of Bio-Techne | Catalog # AF-289-PBR

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Product Datasheet / COA

Key Product Details

Species Reactivity:	Human, Mouse, Rat
Immunogen:	E. coli-derived recombinant human MIF Pro2-Ala115 Accession # AAA36315
Applications:	CyTOF-ready, Flow Cytometry, Western Blot
Label:	Alexa Fluor 647 (Excitation=650 nm, Emission=668 nm)
Specificity:	Detects human, mouse, and rat MIF in Western blots.

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Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
CyTOF-ready	Optimal dilution of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: MIF

MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4-7). Secretion occurs non-classically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50-65 have also been suggested to contain thiol-protein oxidoreductase activity (12). MIF has proinflammatory cytokine activity centered around aa’s 49-65. On fibroblasts, MIF induces, IL-1, IL-8, and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release following IFN-gamma activation (13, 14). MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (15, 16). Human MIF is active on mouse cells (14). Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine, and rat MIF, respectively.

Item	Value
Long Name	Macrophage Migration Inhibitory Factor
Entrez Gene IDs	4282 (Human); 17319 (Mouse)
Alternate Names	EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, MIF, MMIF, Phenylpyruvate tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor)

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