Human/Mouse/Rat HCLS1 Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5789AFP488

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AF5789AFP488-100UG
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Goat IgG

View all HCLS1 Antibodies »

Product Specifications

Specificity

Detects endogenous human, mouse and rat HCLS1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Background: HCLS1

HCLS1 (Hematopoietic lineage cell-specific protein; also HS1 and LckBP1) is a cytoplasmic substrate for non‑receptor type protein‑tyrosine kinases. Although its predicted MW is 54 kDa, it migrates anomalously at 75‑80 kDa in SDS-PAGE. HCLS1 is restricted to myeloid/lymphoid lineage cell types, including platelets, B cells, T cells and mast cells. HCLS1 binds to F-actin and promotes actin polymerization. It also serves as an intermediate for src-mediated signal transduction. Human HCLS1 is 486 amino acids (aa) in length. It contains one HAX1 binding site (aa 27‑66), three-plus cortactin type repeats (aa 79‑212), a coiled‑coil region (aa 215‑280) and an SH3 domain (aa 437‑484). HCLS1 is initially phosphorylated by Syk at Tyr378 and Tyr397, followed by a Src family kinase phosphorylation at Tyr222. This activates HCLS1, allowing it to interact with Ras-associated Grb2.

Long Name

Hematopoietic Cell-specific Lyn Substrate 1

Alternate Names

Cortactin-like, CTTNL, LckBP1

Entrez Gene IDs

3059 (Human); 15163 (Mouse); 288077 (Rat)

Gene Symbol

HCLS1

UniProt

P14317

Additional HCLS1 Products

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Product Datasheets

Certificate of Analysis

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Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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